Neem Oil Fly Spray for Farm Animals

Neem Oil Fly Spray for Farm Animals

Fly spray for horses or cattle can be expensive. Creating your own fly spray by using Neem oil reduces the cost, allows you to tailor the mix to your animal's needs, and is all-natural.  This may be a very effective fly spray.  

  Things You'll Need

  • 2 cups vinegar
  • Water to fill 32oz
  • 1 TBSP. Neem oil (or Neem Extract)*
  • 1/2 tsp. essential oil of garlic or tea tree (optional)**                                            
  • Spray bottle
  • Funnel
  • If a healing effect is needed, replace the vinegar with Aloe Vera Juice and the Garlic EO with Lavender EO.  

*The Neem Oil will separate, so this needs to be shaken before and during each use. The Neem Extract will not separate but is not as effective as the oil.   

**The garlic essential oil will increase the effects of the formula but it works well without also. 


  1. Remove the nozzle from an old fly spray bottle or a commercially bought spray bottle. Insert a funnel.
  2. Pour 2 cups of vinegar into the bottle, using the funnel. You can use either apple-cider, distilled vinegar or for healing use Aloe Vera Juice. Add 1 cup of water.
  3. Add the 1 TBSP Neem Oil. 
  4. Replace the nozzle and shake. It makes about 32 ounces of fly spray, which is the typical size of a bottle of commercial fly spray.


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  • APC Collaborator
Comments 18
  • Zatural

    Hi Melissa, if you’re worried, you can always dilute the mixture more than the recipe recommends. Neem Oil is pretty gentle when diluted properly, though!

  • Melissa Watson
    Melissa Watson

    is this too potent for calves? would they have a reaction to it?

  • Zatural

    Yes, You could do a mixture.

  • Jacque

    Can you do part vinegar and part aloe vera?

  • Zatural

    Hi Sandy, you can add a few drops of neem oil to your animal’s shampoo to kill and repel fleas and ticks. You can also create an all-natural flea spray by combining a dozen drops of neem oil, half-a-dozen drops of dish soap and a cup of warm water.

  • sandy page
    sandy page

    Does it repel ticks?

  • sam newman
    sam newman

    I used undiluted neem oil on my jersy cow for flies, and after a month, she is having significant hair loss in some places, but not others, where I applied it heavily. She has hair loss and irritation on the ears, spine and tail bone. I put the oil all over her included belly, bag, flanks, chest, and not problems there so far. Is it known whether too much neem oil undiluted can cause such an irritation and hair loss? Neem oil is reputed to heal the skin and hair, but perhaps someone knows more about this who can comment on this for me. Thanks, Sam

  • Zatural

    JoAnn, Im so sorry. Thank you for pointing out that error. That is supposed to be filled with water to the 32oz level.

  • JoAnn

    How does 3 cups of fluid equate to 32oz?

  • Zatural

    Lana, Im not sure I understand your question completely and I’d hate to answer wrong. Can you please reach out to our team at or by phone 208-969-1282 and we will make sure to answer your question correctly.

  • Lana Fowler
    Lana Fowler

    How much aloe and water total for the healing one?

  • Zatural

    Stella, to answer you question, it acts a a Repellent and an Insecticide.

  • STella

    Does this merely repel or kill flies

  • STella

    Does this merely repel or kill flies

  • Max

    Do you use white vinegar or apple cider vinegar?

  • Emiley

    Would his be ok for sheep?

  • Zatural

    Larry, yes it will work. Recommendation is to dilute and spray over cattle.

  • Larry Ferguson
    Larry Ferguson

    I will try this on my cows to assist in the treatment of horn fly infestation.

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